Seven Hills International Limited


 Analyser Systems



BIOAqua - Online Automatic E Coli & T Coli Analyser

The Escherichia coli and total coliform parameters are key and essential indicators for determining possible uses and reuse of the water.

RD1620/2007, which sets the legal basis for reusing treated water requires controlling Escherichia coli and depending on its concentration, defines the allowed uses for the water that is regenerated.

In addition, directive 2006/7/CE relative to the management of the quality of bath water classifies the quality of this resource according to several parameters, where the determination of the presence of Escherichia coli is an important factor.

BIOAqua - Online Automatic E Coli & T Coli Analyser is especially designed for determining the total Escherichia coli and coliforms in water, which makes it an optimum tool for water monitoring according to its use.


BIOAqua - Online Automatic E Coli & T Coli Analyser – Operation

Measurement Principle

The measurement principle is based on DST® (defined substrate technology), with an alternate fluorescence and absorbance detection system.

Defined Substrate Technology BIOAqua - Online Automatic E Coli & T Coli Analyser uses reagent Colilert-18® (*) based on defined substrate technology (DST®) for the detection of total coliforms and Escherichia coli.

The coliforms use the β-galactosidase to metabolise ONPG and generate a yellow colour in the medium.

Escherichia coli, also uses β-glucuronidase to metabolise MUG and generate fluorescence.

The determination of E. coli as well as total coliforms is based on the correlation that exists between the concentration of bacteria and the time when the fluorescence and/or the colour appears.

The sensitivity of the system allows detecting concentrations of 108 NMP after 3 hours.

Disinfection of the System

The independence between samples is assured by an adequate hydraulic and mechanical management of the system, avoiding contamination between the different samples


BIOAqua - Online Automatic E Coli & T Coli Analyser - Specifications
  • Hydraulic and fluid management system.
  • Thermostatic measuring chamber that includes the bucket where the incubation and measurement of the sample is conducted optically.
  • Multi-λ optic measuring system.
  • Software and electronics capable of controlling the system and managing the data.


Unique Characteristics of BIOAqua - Online Automatic E Coli & T Coli Analyser
  • Simultaneous measurement of Escherichia coli and total coliforms.
  • Calculating the NMP in 3 h for waters that are highly contaminated.
  • Low maintenance.
    The system only requires maintenance every 15 days. These quarterly tasks are limited to replacing reagents and a monthly semi-automatic cleaning and replacement of a consumable part.
  • Programming the equipment.
    BIOAqua - Online Automatic E Coli & T Coli Analyser is programmable and allows carrying out daily analysis at the same time or consecutively, providing a graphic display of the accumulated values.
  • Transmission to the control centre.


The data may be transmitted to a control centre automatically for analysis and use. Furthermore, BIOAqua - Online Automatic E Coli & T Coli Analyser operates the results and sends alarms to other equipments (ie. BIOAqua Online Auto Sampler, the automatic sampling equipment) or to additional water monitoring networks.


Benefits of BIOAqua - Online Automatic E Coli & T Coli Analyser
  • Analyser is used to determine the status of process and product water quality in Food, Pharmaceutical industries, the results in 180 minutes reduces the waiting period substantially enabling the plant operators to make fast decision on their plant operations

  • The system is used determine the quality of water in rivers for safe bath usage in accordance with 2006/7/CE

  • This system can help identify the pollution issues relating to presence of ammonia in the waste water stream as presence of ammonia in

  • Effluents can discharged based on line E coli values, this enable industries to operate with the regulation and can avoid penalties

BIOAqua - Online Automatic E Coli & T Coli Analyser - Specifications


Measurement principle:

-Fluorimetric detection for Escherichia coli

-Colorimetric detection for total coliforms

Reagents used:

-Colilert-18® (*) from IDEXX in liquid form

-Cleaning solution

Detection limit:

-Escherichia coli: 1 bacteria in 100 ml

-Total coliforms: 1 bacteria in 100 ml

Temperature regulation: ±0,1 º C
Analysis time: - from 3 to 18 hours, depending on the concentration
Power supply: - 110 – 230 VAC/50 -60 Hz

- RS-232 port and RS-485 port

- MOD-BUS or ASCII protocol

- Analogue output 0-10 V/4-20 mA

- Alphanumeric terminal graph in colour

- Data extraction program via PC

Alarms: - Two alarms, which may be upgraded to four

-105 x 70 x 42 cm


(*) Colilert-18 ® is a registered trademark. The reagent liquid form has been developed by our partners, without IDEXX validation.
BIOAqua - Online Automatic E Coli & T Coli Analyser – Software

BIOAquaView, the desktop-based data download application which allows data download, visualization and export in a user-friendly environment. The software allows tracking events such as sampling status or cleanups. BIOAquaView is the perfect complement to your BIO Aqua range of products